Data Sheet
Omalizumab (Xolair®) – PK ELISA
Catalogue Number: SB-07-032
For the quantitative determination of Omalizumab in human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| Detection Method | Colorimetric; absorbance at 450 nm with a reference at 650 nm.. |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Calibrator | Xolair® |
| Sensitivity | Detection limit of 39 ng/mL. |
| Precision | Intra-assay precision ranges from 4 to 11%. Inter-assay mean precision is 13%. Determined by analysis of samples at 500 ng/mL in 6 replicates, on 6 different occasions. |
| Accuracy | Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. The Mean %bias is ≤ 20%. |
| Total Error | Calculated by adding the mean %bias with inter-assay variability (%CV). The Total Error is ? 24% |
| Hook Effect | No hook effect was observed up to 40,000 ng/mL of omalizumab. |
| Dilutional Linearity | Dilutional linearity was established up to 800 fold. Samples can be diluted up to 800-fold without any significant impact on the recovery of the samples. |
| Interpretation of Results | A calibration curve is constructed by plotting the absorbance values on a 4-point parameter fit curve. Concentration of omalizumab in test samples is interpolated from the calibration curve. |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
