Somru Bioscience

Mouse Anti-Etanercept (Enbrel®) Antibody

$499.00

Catalog #: SB-01-112

SKU: SB-01-112 Category: Tags: , ,
Clonality

Hosts

Conjugate

Size

0.1 mg

Data Sheet

Mouse Anti-Etanercept (Enbrel®) Antibody, 0.1 mg

Catalogue Number: SB-01-112

Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.

Product Specifications Data Sheet

About Mouse monoclonal antibody against etanercept (Enbrel®)
Quantity 0.1 mg
Host/ Isotype Mouse monoclonal IgG1
Immunogen Human etanercept (Enbrel®)
Purification Protein G purification from hybridoma culture supernatant
Buffer Phosphate buffered saline (PBS) with trehalose
Specificity This antibody binds to recombinant human etanercept (Enbrel®). It detects human TNF RII in ELISAs and Western blots. No cross reactivity was observed with recombinant human (rh) TNF RI, rhTNF?, or rhTNF?.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS
Storage Store lyophilized antibody at -20 to -70°C for one year from date of receipt. Once reconstituted, store the antibody undiluted at 2 to 8°C for one month, or in aliquots at -20 to -70 °C for six months. Avoid repeated freezing and thawing.
Applications
Purpose Recommended Concentration Comment
ELISA- Coating antibody 2- 8 µg/mL To capture etanercept by ELISA
ELISA- Detection antibody 0.1- 0.4 µg/mL To detect etanercept by ELISA
Neutralization 0.5- 2 ?g/mL To neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in appropriate cell line
Western Blot 1 µg/mL To detect etanercept in non-reducing condition
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Safety/ Precautions It should be handled by trained personnel only.  Please contact tec@somrubioscience.com for more details.

                                                        FOR RESEARCH USE ONLY

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