Data Sheet
Insulin Aspart (Novolog®) – PK ELISA
Catalogue Number: SB-07-052
For the quantitative determination of Insulin Aspart in human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Insulin Aspart PK ELISA is a sandwich enzyme immunoassay in which two monoclonal antibodies are directed against separate epitopes on the Aspart molecule. |
| Detection Method | Colorimetric |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Calibrator | Recombinant human insulin |
| Assay range | 3-100 µU/mL |
| Sensitivity | 3 µU/mL |
| Precision | Intra-assay precision ranges from 5 to 9%. Inter-assay mean precision is 11%. Determined by analysis of samples at 20 µU/mL in 6 replicates, on 6 different occasions. |
| Accuracy | Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. The Mean %bias is ≤ 20%. |
| Total Error | Calculated by adding the mean %bias with inter-assay variability (%CV). The Total Error is ≤ 20% |
| Cross reactivity | Insulin 100 % C-peptide < 0.1 % Proinsulin 54 % Insulin lispro 102 % Insulin aspart 100 % Insulin determir 25 % Insulin glargine 45 % Insulin glulisine 111 % Rat insulin 75 % Mouse insulin 54 % Porcine insulin 71 % Ovine insulin 108 % Bovine insulin 48 % |
| Interpretation of Results | A calibration curve is constructed by plotting the absorbance values on a 4-point parameter fit curve. The concentration of insulin aspart in test samples is interpolated from the calibration curve. |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents are handled by trained laboratory staff and are used in accordance with GLP. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
