Somru Bioscience

Donkey Anti-Chicken IgY Antibody (HRP)

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Catalog #: SB-02-484

SKU: SB-02-484 Category: Tags: ,
Clonality

Hosts

Data Sheet

Donkey Anti-Chicken IgY Antibody (HRP)

Catalogue Number: SB-02-484

Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.

Product Specifications Data Sheet

About Donkey polyclonal antibody against chicken IgY- HRP conjugate
Detection Method Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm
Quantity 0.4 mL
Host/Isotype Donkey polyclonal IgG
Immunogen Chicken IgY
Purification Affinity purified and conjugated to horseradish peroxidase
Storage Store the antibody undiluted at -20 to -70 °C for six months from date of receipt. Avoid repeated freezing and thawing.
Positive Control Affinity purified Rabbit anti-AAV1 antibodies
Specificity This antibody binds to heavy and light chain of chicken IgY. No cross reactivity to human, mouse or rat serum proteins
Buffer BSA in Phosphate Buffer Saline (PBS)
Reconstitution Not applicable
Applications
Purpose Recommended Concentration Comment
ELISA- detection antibody 1:10,000 To detect chicken IgY by ELISA
Western Blot 1:50,000 To detect chicken IgY
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Safety/ Precautions It should be handled by trained personnel only.  Please contact tec@somrubioscience.com for more details.
Statistical Cut Point We strongly recommend each lab develop their own statistical cutpoint in the appropriate study population using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281). For further details please contact tec@somrubioscience.com. for more details.

 

Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.

                FOR RESEARCH USE ONLY

Donkey Anti-Chicken IgY Antibody (HRP)

Catalogue Number: SB-02-484

 

Product Specifications Data Sheet

Description Donkey polyclonal antibody against chicken IgY- HRP conjugate
Quantity 0.4 mL
Host/Isotype Donkey polyclonal IgG
Immunogen Chicken IgY
Purification Affinity purified and conjugated to horseradish peroxidase
Buffer BSA in Phosphate Buffer Saline (PBS)
Specificity This antibody binds to heavy and light chain of chicken IgY. No cross reactivity to human, mouse or rat serum proteins
Reconstitution Not applicable
Storage Store the antibody undiluted at -20 to -70 °C for six months from date of receipt. Avoid repeated freezing and thawing.
Applications Purpose Recommended Concentration Comment
ELISA- detection 1:10,000 To detect chicken IgY by ELISA
Western Blot 1:50,000 To detect chicken IgY
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Safety/ Precautions It should be handled by trained personnel only.  Please contact our customer service at customer@somrubioscience.com for more details.

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