Data Sheet
CRISPR/Cas9 ELISA
Catalogue Number: SB-07-0108
For the quantitative determination of CRISPR/CAS9 from whole cell extracts, serum, or plasma.
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® CRIPSR/CAS9 ELISA kit is a double antibody sandwich immunoassay. The kit utilizes a polyclonal antibody as a capture reagent and another polyclonal antibody for the detection. The use of polyclonal antibodies allows for the detection of both native and recombinant Cas9. |
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Calibrator | Recombinant Cas9 |
| Calibration Curve | 10 – 1000 ng/mL |
| Assay Range | 10 – 1000 ng/mL |
| Sensitivity | 10 ng/mL |
| Precision | Intra-assay precision ≤ 15%. Inter-assay mean precision ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Accuracy | The Mean %bias is ≤ 10%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Total Error | The Total Error is ≤ 20%. Calculated by adding the mean %bias with inter-assay variability (%CV). |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
