1 pack ( 2 x 96 wells)
C1-inhibitor (Berinert®) PK
Catalog #: SB-07-023
C-1 Esterase Inhibitor(Berinert®) Activity Assay
Catalogue Number: SB-07-023
Notice: This product specification data sheet is for information purposes only. Please contact firstname.lastname@example.org for up to date information.
Product Specifications Data Sheet
|The Somru® C-1 inhibitor(Berinert®) activity assay kit utilizes the ability of the C esterase inhibitor enzyme to inhibit recombinant human complement component C1s and subsequent cleavage of a colorimetric peptide substrate.
This assay can be used to measure endogenous C1 inhibitor and for monitoring substitution therapy and steroid therapy in plasma.
|Colorimetric; Assay can be read as kinetic (ΔA405 nm/minute) or endpoint (A405 nm) method.
|Store the unopened kit at 2-8 °C.
|Quality Control Samples (QCs)
|Three levels of QCs are included for custom GLP kits. QCs are prepared by fortifying human plasma derived C1-esterase inhibitor in human plasma or serum.
|The precision was determined by analyzing samples prepared at 100nM in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation (CV)) ranges from 2% to 7%. Inter-assay precision was at 9%.
|100nM C1-inhibitor was fortified in 10 lots of human serum. Recovery ranges are from 91-109% with an average recovery of 104%.
|Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be less than 13%.
|The limit of detection is 0.8 nM.
|Samples can be diluted up to 32 fold. Assay range may be changed to meet intended purpose by changing ratio of sample and C1s. Please contact our technical service team at email@example.com.
|No known cross-reactivity exists.
|Safety Warnings and Precautions
|All chemicals should be considered potentially hazardous. We recommend that this product and its components be handled by those trained in laboratory techniques and be used according to the principles of Good Laboratory Practice.
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY