Somru BioScience

Anti-CRISPR/Cas9 Antibody ELISA


Catalog #: SB-06-0107

SKU: SB-06-0107 Category: Tag:

Data Sheet

Anti-CRISPR/Cas9 Antibody ELISA

Catalogue Number: SB-06-0107

For the semi-quantitative determination of anti-Cas9 antibodies in serum or plasma.

Notice: This product specification data sheet is for information purposes only. Please contact for up to date information.

Product Specifications Data Sheet

About The Somru® anti-Cas9 antibody ELISA kit is a sandwich immunoassay method. The Cas9 protein is immobilized to the plate to capture anti-Cas9 antibodies and an anti-species antibody is for detection. The assay is capable of detecting both IgG and IgM. The assay can be utilized as a screening ELISA to classify samples as positive or negative or it can be used for the determination of anti-Cas9 antibody titer. The assay is compatible for both serum and plasma from various species.
Please contact for information specific to your species.
Detection Method Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm
Storage Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival.
Positive Control Affinity purified Rabbit anti-CRISPR/Cas9 antibodies
Sensitivity Less than 50 ng/mL of rabbit ant-CRISPR/Cas9 antibodies
Precision Intra-assay precision ≤ 18%. Inter-assay mean precision ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions.
Relative Accuracy The Mean %bias is ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions.
Statistical Cut Point We strongly recommend each lab develop their own statistical cutpoint in the appropriate study population using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281). For further details please contact


Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.

                FOR RESEARCH USE ONLY





0.1 mg

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