Data Sheet
Pertuzumab (Perjeta®) – PK ELISA
Catalogue Number: SB-07-078
For the quantitative determination of Pertuzumab in human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Pertuzumab ELISA kit is designed to measure pharmacologically relevant Pertuzumab with high specificity and enhanced sensitivity in serum or plasma. This user-friendly assay design utilizes target protein, recombinant HER2-extracellular domain (ECD), as capture reagent and anti-Pertuzumab antibody for detection.
If you intend to measure total pertuzumab (Her-2 bound and free) or intend to measure mertuzumab in presence of trastuzumab (Herceptin®), please contact tec@somrubioscience.com for additional information. |
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm. |
| Storage | Kit ships at 2-8 °C. Multiple storage temperatures upon arrival. |
| Calibrator | Pertuzumab (Supplied by client) |
| Calibration Curve | 62.5 to 8,000 ng/mL; 4 or 5 -parameter curve fit |
| Assay Range | 125 to 4000 ng/mL |
| Sensitivity | 125 ng/mL |
| Precision | Intra-assay precision and inter-assay mean precision ? 20%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Accuracy | The Mean %bias is ? 20% (except at ? 25% at lower limit of quantitation). Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Total Error | The total error is ? 25%. Calculated by adding the mean %bias with inter-assay variability (%CV). |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potnetially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personell abd are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation pf reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
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