Data Sheet
Glucagon(GlucaGen®) PK ELISA
Catalogue Number: SB-07-014
For the quantitative determination of Glucagon in human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Glucagon ELISA assay employs the quantitative sandwich enzyme immunoassay technique using two monoclonal antibodies specific for pancreatic glucagon. This user-friendly assay design utilizes a pair of antibodies that allows the detection of the whole pancreatic glucagon molecule in biological matrices with minimal or no cross reactivity to other form of glucagon molecules. | ||||||||||
| Detection method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm. | ||||||||||
| Storage | Store the unopened kit at 2-8 °C. | ||||||||||
| Safety Warnings and Precautions | All chemicals should be considered potentially hazardous. We recommend that this product and its components be handled by those trained in laboratory techniques and be used according to the principles of Good Laboratory Practice. | ||||||||||
| Quality Control Samples (QCs) | Three levels of QCs are included for custom GLP kits for an additional cost. QCs are prepared by fortifying glucagon in human serum or plasma. | ||||||||||
| Precision | The precision was determined by analyzing samples prepared at 450 pg/mL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 3% to 7%. Inter-assay precision was at 9%. | ||||||||||
| Accuracy | The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean %bias was less than 12%. | ||||||||||
| Total Error | Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be less than 15%. | ||||||||||
| Selectivity/ Recovery | The recovery of glucagon was evaluated by fortifying 200 pg/mL of glucagon in various matrices. The recovery ranged from 91% to 107%. | ||||||||||
| Sensitivity | The limit of detection is 11.7 pg/mL. | ||||||||||
| Dilutional Linearity | Dilutional linearity was established up to a dilution of 16 fold. The assay is capable of measuring samples with up to 32000 pg/mL of glucagon. | ||||||||||
| Specificity |
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Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
