Data Sheet
Erythropoietin (Epogen®) – PK ELISA
Catalogue Number: SB-07-020
For the quantitative determination of Erythropoietin in human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Erythropoietin ELISA assay employs the quantitative double antibody sandwich enzyme immunoassay technique using two monoclonal antibodies specific for erythropoietin. This user-friendly assay design utilizes a pair of antibodies that allows the detection of the erythropoietin molecule in biological matrices with minimal or no cross reactivity to other form of erythropoietin molecules. |
| Detection method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm. |
| Storage | Store the unopened kit at 2-8 °C. |
| Quality Control Samples (QCs) | Three levels of QCs are included for custom GLP kits for an additional cost. QCs are prepared by fortifying erythropoietin in human serum or plasma. |
| Precision | The precision was determined by analyzing samples prepared at 50 mIU/mL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 8% to 11%. Inter-assay precision was at 15%. |
| Accuracy | The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean %bias was less than 7%. |
| Total Error | Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be less than 22%. |
| Selectivity/ Recovery | The recovery of erythropoietin was evaluated by fortifying 50 mIU/mL of erythropoietin in various matrices. The recovery ranged from 90% to 101%. |
| Sensitivity | The limit of detection is 2 mIU/mL. |
| Dilutional Linearity | Dilutional linearity was established up to a dilution of 20 fold. |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
