Data Sheet
Donkey Anti-Chicken IgY Antibody (HRP)
Catalogue Number: SB-02-484
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | Donkey polyclonal antibody against chicken IgY- HRP conjugate | ||||||||||
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm | ||||||||||
| Quantity | 0.4 mL | ||||||||||
| Host/Isotype | Donkey polyclonal IgG | ||||||||||
| Immunogen | Chicken IgY | ||||||||||
| Purification | Affinity purified and conjugated to horseradish peroxidase | ||||||||||
| Storage | Store the antibody undiluted at -20 to -70 °C for six months from date of receipt. Avoid repeated freezing and thawing. | ||||||||||
| Positive Control | Affinity purified Rabbit anti-AAV1 antibodies | ||||||||||
| Specificity | This antibody binds to heavy and light chain of chicken IgY. No cross reactivity to human, mouse or rat serum proteins | ||||||||||
| Buffer | BSA in Phosphate Buffer Saline (PBS) | ||||||||||
| Reconstitution | Not applicable | ||||||||||
| Applications |
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| Safety/ Precautions | It should be handled by trained personnel only. Please contact tec@somrubioscience.com for more details. | ||||||||||
| Statistical Cut Point | We strongly recommend each lab develop their own statistical cutpoint in the appropriate study population using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281). For further details please contact tec@somrubioscience.com. for more details. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
Donkey Anti-Chicken IgY Antibody (HRP)
Catalogue Number: SB-02-484
Product Specifications Data Sheet
| Description | Donkey polyclonal antibody against chicken IgY- HRP conjugate | ||
| Quantity | 0.4 mL | ||
| Host/Isotype | Donkey polyclonal IgG | ||
| Immunogen | Chicken IgY | ||
| Purification | Affinity purified and conjugated to horseradish peroxidase | ||
| Buffer | BSA in Phosphate Buffer Saline (PBS) | ||
| Specificity | This antibody binds to heavy and light chain of chicken IgY. No cross reactivity to human, mouse or rat serum proteins | ||
| Reconstitution | Not applicable | ||
| Storage | Store the antibody undiluted at -20 to -70 °C for six months from date of receipt. Avoid repeated freezing and thawing. | ||
| Applications | Purpose | Recommended Concentration | Comment |
| ELISA- detection | 1:10,000 | To detect chicken IgY by ELISA | |
| Western Blot | 1:50,000 | To detect chicken IgY | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application. | |||
| Safety/ Precautions | It should be handled by trained personnel only. Please contact our customer service at customer@somrubioscience.com for more details. | ||
