Data Sheet
Denosumab (Prolia®) – PK ELISA
Catalogue Number: SB-07-064
For the quantitative determination of Denosumabin human serum and plasma
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® denosumab ELISA kit is designed to measure denosumab with high specificity and enhanced sensitivity in serum or plasma. This user-friendly assay design utilizes target protein (RANKL) to capture and anti-denosumab antibody for detection. |
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Calibrator | Denosumab |
| Calibration Curve | 25 to 2500 ng/mL; 4 or 5 -parameter curve fit |
| Assay Range | 25 to 1,000 ng/mL |
| Sensitivity | 25 ng/mL |
| Precision | Intra-assay precision ≤ 10%. Inter-assay mean precision ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Accuracy | The Mean %bias is ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Total Error | The Total Error is ≤ 20%. Calculated by adding the mean %bias with inter-assay variability (%CV). |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
