Data Sheet
Bevacizumab (Avastin®) – PK ELISA
Catalogue Number: SB-07-041
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Bevacizumab ELISA kit is designed to measure bevacizumab with high specificity and enhanced sensitivity in serum or plasma. This user-friendly assay design utilizes bevacizumab specific anti-idiotype monoclonal antibody to capture bevacizumab to the plate and bevacizumab specific polyclonal antibody for detection. |
| Detection method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm. |
| Storage | Store the unopened kit at 2-8 °C. |
| Quality Control Samples (QCs) | Three levels of QCs are included for custom GLP kits for an additional cost. Custom GLP kits includes three levels of QCs. QCs are prepared by fortifying bevacizumab in human serum. |
| Precision | The precision was determined by analyzing samples prepared at 1000 ng/mL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 2% to 6%. Inter-assay precision was at 5%. |
| Accuracy | The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean absolute %bias was less than 4%. |
| Total Error | Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be 9%. |
| Selectivity Recovery | Selectivity of the assay was evaluated by spiking 10 individual serum lots with 1000 ng/ml of Bevacizumab. The recovery ranges from 99% to 111%. The mean recovery was 103%. |
| Sensitivity | The limit of detection is 82 ng/mL. |
| Dilutional Linearity | Dilutional linearity was established up to 40 fold. |
| Hook Effect | No hook effect was observed up to 200 µg/mL of bevacizumab. |
| Sample Pre-treatment (ADA Interference) | If a high level of anti-bevacizumab antibody is expected to be present in test samples, an acid dissociation step may be incorporated to minimize the impact of these antibodies in bevacizumab quantitation. Please contact our technical service team at tec@somrubioscience.com. |
| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
