Data Sheet
Anti-AAV9 Antibody ELISA
Catalogue Number: SB-06-0106
For the semi-quantitative determination of anti-AAV9 antibodies.
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® anti-AAV9 antibody ELISA kit is a sandwich immunoassay method. The AAV9 vector particle is immobilized to the plate to capture anti-AAV9 antibodies and anti-species antibody is used for detection. The assay is capable of detecting both IgG and IgM. The assay can be utilized as a screening ELISA to classify samples as positive or negative or it can be used for the determination of anti-AAV9 antibody titer. The assay is compatible for both serum and plasma from various species. Please contact tec@somrubioscience.com for information specific to your species. |
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Positive Control | Affinity purified Rabbit anti-AAV9 antibodies |
| Sensitivity | Less than 20 ng/mL of rabbit anti-AAV9 antibodies |
| Precision | Intra-assay precision ≤ 18%. Inter-assay mean precision ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Relative Accuracy | The Mean %bias is ≤ 16%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Statistical Cut Point | We strongly recommend each lab develop their own statistical cutpoint in the appropriate study population using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281). For further details please contact tec@somrubioscience.com. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
